000 | 02699na a2200241 4500 | ||
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003 | PC13155 | ||
005 | 20210625062812.0 | ||
008 | 130622s2013 | ||
040 | _cH12O | ||
041 | _aeng | ||
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_aFernández Moreno, Miguel Ángel _9860 _eInstituto de Investigación i+12 |
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_aHernández, Rosana _9861 _eInstituto de Investigación i+12 |
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_9862 _aGaresse, Rafael _eInstituto de Investigación i+12 |
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245 | 0 | 0 |
_aDrosophila nuclear factor DREF regulates the expression of the mitochondrial DNA helicase and mitochondrial transcription factor B2 but not the mitochondrial translation factor B1 _h[artículo] |
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_bBiochimica et Biophysica Acta, _c2013. |
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300 | _a1829(10):1136-1146. | ||
500 | _aFormato Vancouver: Fernández Moreno MA, Hernández R, Adán C, Roberti M, Bruni F, Polosa PL, et al. Drosophila nuclear factor DREF regulates the expression of the mitochondrial DNA helicase and mitochondrial transcription factor B2 but not the mitochondrial translation factor B1. Biochim Biophys Acta. 2013;1829(10):1136-46. | ||
504 | _aContiene 40 referencias, 5 figuras y 1 tabla. | ||
520 | _aResumen: DREF [DRE (DNA replication-related element)-binding factor] controls the transcription of numerous genes in Drosophila, many involved in nuclear DNA (nDNA) replication and cell proliferation, three in mitochondrial DNA (mtDNA) replication and two in mtDNA transcription termination. In this work, we have analysed the involvement of DREF in the expression of the known remaining genes engaged in the minimal mtDNA replication (d-mtDNA helicase) and transcription (the activator d-mtTFB2) machineries and of a gene involved in mitochondrial mRNA translation (d-mtTFB1). We have identified their transcriptional initiation sites and DRE sequences in their promoter regions. Gel-shift and chromatin immunoprecipitation assays demonstrate that DREF interacts in vitro and in vivo with the d-mtDNA helicase and d-mtTFB2, but not with the d-mtTFB1 promoters. Transient transfection assays in Drosophila S2 cells with mutated DRE motifs and truncated promoter regions show that DREF controls the transcription of d-mtDNA helicase and d-mtTFB2, but not that of d-mtTFB1. RNA interference of DREF in S2 cells reinforces these results showing a decrease in the mRNA levels of d-mtDNA helicase and d-mtTFB2 and no changes in those of the d-mtTFB1. These results link the genetic regulation of nuclear DNA replication with the genetic control of mtDNA replication and transcriptional activation in Drosophila. | ||
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_9625 _aInstituto de Investigación imas12 |
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_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc13155.pdf _ySolicitar documento |
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_2ddc _cART _n0 |
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_c13155 _d13155 |