000			nab a22 7a 4500
999			_c16045 _d16045
003			PC16045
005			20210625062819.0
008			200709b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_9726 _aRubio Valladolid, Gabriel _ePsiquiatría
245	0	0	_aEffects of naltrexone plus topiramate on ethanol self-administration and tyrosine hydroxylase gene expression changes. _h[artículo]
260			_bAddiction biology, _c2014
300			_a19(5):862-73.
500			_aFormato Vancouver: Navarrete F, Rubio G, Manzanares J. Effects of naltrexone plus topiramate on ethanol self-administration and tyrosine hydroxylase gene expression changes. Addict Biol. 2014 Sep;19(5):862-73.
501			_aPMID: 23573810
504			_aContiene 55 referencias
520			_aThe purpose of this study was to evaluate the effects of naltrexone (0.7 mg/kg) and/or topiramate (25 mg/kg) on ethanol consumption and the motivation to drink in an oral-operant conditioning paradigm in C57BL/6 mice. Subsequent real-time polymerase chain reaction (PCR) experiments were performed to analyze gene expression changes in tyrosine hydroxylase (TH) in the ventral tegmental area (VTA). The administration of naltrexone significantly reduced ethanol consumption and the motivation to drink during the different stages of the experiment, whereas the treatment with topiramate resulted in a much lower effect. Interestingly, the administration of naltrexone plus topiramate reduced ethanol consumption markedly compared with single-drug treatment. The water self-administration paradigm was also performed using the same drugs and no differences were found between treatment groups. Real-time PCR analyses revealed that naltrexone significantly normalized the increase of TH gene expression in the VTA induced by ethanol, whereas the administration of topiramate did not produce any significant effect. In the ethanol self-administration procedure, the combination of both drugs further reduced TH gene expression, reaching statistical significance compared with the vehicle, naltrexone or topiramate groups. Taken together, these findings indicate that the administration of naltrexone plus topiramate further reduced ethanol consumption and the motivation to drink in comparison with single-drug treatment. This action may be due, at least in part, to a greater decrease in TH gene expression in the VTA. These results suggest that the combination of both drugs deserves further exploration for the treatment of problems related to alcohol consumption.
710			_9150 _aServicio de Psiquiatría
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc16045.pdf _ySolicitar documento
942			_2ddc _cART _n0