000			nab a22 7a 4500
999			_c16577 _d16577
003			PC16577
005			20210811104727.0
008			210804b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_91616 _aViedma Moreno, Esther _eInstituto de Investigación i+12
100			_91506 _aSanz Sanz, Francisca _eMicrobiología y Parasitología
100			_91619 _aOrellana Miguel, María Ángeles _eMicrobiología y Parasitología
100			_9869 _aSan Juan Garrido, Rafael _eMedicina Interna
100			_9876 _aAguado García, José María _eEnfermedades Infecciosas
100			_9868 _aRodríguez Otero, Joaquín _eMicrobiología y Parasitología
100			_978 _aChaves Sánchez, Fernando _eMicrobiología y Parasitología
245	0	0	_aRelationship between agr dysfunction and reduced vancomycin susceptibility in methicillin-susceptible Staphylococcus aureus causing bacteraemia. _h[artículo]
260			_bThe Journal of antimicrobial chemotherapy, _c2014
300			_a69(1):51-8.
500			_aFormato Vancouver: Viedma E, Sanz F, Orellana MA, San Juan R, Aguado JM, Otero JR et al. Relationship between agr dysfunction and reduced vancomycin susceptibility in methicillin-susceptible Staphylococcus aureus causing bacteraemia. J Antimicrob Chemother. 2014 Jan;69(1):51-8.
501			_aPMID: 23975741
504			_aContiene 38 referencias
520			_aObjectives: Limited data exist regarding the role of agr dysfunction in reducing susceptibility to vancomycin in methicillin-susceptible Staphylococcus aureus (MSSA). This study investigated the clinical and molecular epidemiology of MSSA causing bacteraemia, with emphasis on the reduced susceptibility to vancomycin (RSV) phenotype (MIC ≥ 1.5 mg/L) and its relationship with agr dysfunction. Methods: All MSSA bloodstream isolates obtained at our hospital during 2010 were analysed. Antimicrobial susceptibility was determined and time-kill experiments were performed for oxacillin. Multilocus sequence type and agr genotype were determined and DNA microarray analysis of virulence factors was performed. agr dysfunction was assessed phenotypically and by RT-PCR quantification of RNAIII. Results: Of 84 MSSA, 55 (65.5%) exhibited the RSV phenotype, comprising 13 clonal complexes. agr II polymorphism was more prevalent in RSV than non-RSV isolates (41.8% versus 17.2%, P = 0.023) and average levels of RNAIII gene expression were higher in RSV than non-RSV isolates (ΔCt 4.05 ± 3.29 versus 1.5 ± 2.11, P = 0.005), implying greater agr dysfunction in RSV MSSA. Conclusions: We demonstrated a correlation between RSV phenotype in MSSA and reduced agr expression, particularly in association with the agr II genotype. These results may help to understand the role of agr dysfunction in the increased mortality in MSSA infections.
710			_981 _aServicio de Microbiología y Parasitología
710			_96 _aServicio de Medicina Interna
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc16577.pdf _ySolicitar documento
942			_2ddc _cART _n0