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040			_cH12O
041			_aeng
100			_92943 _aCerveira, Joana F _eInstituto de Investigación imas12
245	0	0	_aShort-term exposure of nontumorigenic human bronchial epithelial cells to carcinogenic chromium(VI) compromises their respiratory capacity and alters their bioenergetic signature. _h[artículo]
260			_bFEBS open bio, _c2014
300			_a4:594-601.
500			_aFormato Vancouver: Cerveira JF, Sánchez Aragó M, Urbano AM, Cuezva JM. Short-term exposure of nontumorigenic human bronchial epithelial cells to carcinogenic chromium(VI) compromises their respiratory capacity and alters their bioenergetic signature. FEBS Open Bio. 2014 Jun 26;4:594-601.
501			_aPMID: 25161867 PMC4141194
504			_aContiene 54 referencias
520			_aPrevious studies on the impact of hexavalent chromium [Cr(VI)] on mammalian cell energetics revealed alterations suggestive of a shift to a more fermentative metabolism. Aiming at a more defined understanding of the metabolic effects of Cr(VI) and of their molecular basis, we assessed the impact of a mild Cr(VI) exposure on critical bioenergetic parameters (lactate production, oxygen consumption and intracellular ATP levels). Cells derived from normal human bronchial epithelium (BEAS-2B cell line), the main in vivo target of Cr(VI) carcinogenicity, were subjected for 48 h to 1 μM Cr(VI). We could confirm a shift to a more fermentative metabolism, resulting from the simultaneous inhibition of respiration and stimulation of glycolysis. This shift was accompanied by a decrease in the protein levels of the catalytic subunit (subunit β) of the mitochondrial H+-ATP synthase (β-F1-ATPase) and a concomitant marked increase in those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The corresponding alteration in the β-F1-ATPase/GAPDH protein ratio (viewed as a bioenergetic signature) upon Cr(VI) exposure was in agreement with the observed attenuation of cellular respiration and enhancement of glycolytic flux. Altogether, these results constitute a novel finding in terms of the molecular mechanisms of Cr(VI) effects. Abbreviations: Cr(III), trivalent chromium; Cr(IV), tetravalent chromium; Cr(V), pentavalent chromium; Cr(VI), hexavalent chromium; DCF, 2′,7′-dichlorofluorescein; 2-DG, 2-deoxyglucose; 2,4-DNP, 2,4-dinitrophenol; EDTA, ethylenediaminetetracetic acid; ETC, mitochondrial electron transport chain; β-F1-ATPase, catalytic subunit (subunit β) of the mitochondrial H+-ATP synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IARC, International Agency for Research on Cancer; OCR, oxygen consumption rate; OXPHOS, oxidative phosphorylation; PBS, phosphate-buffered saline; PI, propidium iodide; ROS, reactive oxygen species; TCA, tricarboxylic acid Keywords: Chromate lung cancer, Warburg effect, Aerobic glycolysis, Cellular respiration, Cellular bioenergetic index, Cellular energy status
710			_9625 _aInstituto de Investigación imas12
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141194/ _yAcceso libre
942			_2ddc _cART _n0