000			na a22 7a 4500
999			_c16788 _d16788
003			PC16788
005			20220328114637.0
008			220325b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_92806 _aMartínez Fernández, Mónica _eInstituto de Investigación i+12
100			_92807 _aDueñas, Marta _eInstituto de Investigación i+12
100			_92996 _aSegovia, Cristina _eInstituto de Investigación imas12
100			_92997 _aRubio, Carolina _eInstituto de Investigación imas12
100			_93022 _aFernández, María _eInstituto de Investigación imas12
100			_9485 _aVillacampa Aubá, Felipe _eUrología
100			_91980 _aDuarte Ojeda, José Manuel _eUrología
100			_92998 _aLópez Calderón, Fernando F _eInstituto de Investigación imas12
100			_92810 _aGómez Rodríguez, María José _eUrología
100			_9882 _aCastellano, Daniel _eOncología Médica
100			_91795 _aRosa Kehrman, Federico de la _eUrología
100			_91219 _aRodríguez Peralto, José Luis _eAnatomía Patológica
100			_92814 _aParamio, Jesús M. _eInstituto de Investigación i+12
245	0	0	_aAnalysis of the Polycomb-related lncRNAs HOTAIR and ANRIL in bladder cancer. _h[artículo]
260			_bClinical epigenetics, _c2015
300			_a7:109.
500			_aFormato Vancouver: Martínez Fernández M, Feber A, Dueñas M, Segovia C, Rubio C, Fernandez M et al. Analysis of the Polycomb-related lncRNAs HOTAIR and ANRIL in bladder cancer. Clin Epigenetics. 2015 Oct 8;7:109.
501			_aPMID: 26457124 PMC4599691
504			_aContiene 27 referencias
520			_aBackground: Long non-coding RNAs (lncRNAs) have been claimed as key molecular players in gene expression regulation, being involved in diverse epigenetic processes. They are aberrantly expressed in various tumors, but their exact role in bladder cancer is still obscure. We have recently found a major role of the Polycomb repression complex in recurrence of non-muscle-invasive bladder cancer. Here, we report the xpression of Polycomb-related lncRNAs:antisense noncoding RNA in the INK4 locus (ANRIL) and HOX antisense intergenic RNA (HOTAIR) in these tumors. Findings: We studied a dataset of non-invasive bladder cancer samples by quantitative reverse transcription PCR (RT-qPCR) and analyzed also invasive bladder cancer samples using TCGA data. Our results showed that, while ANRIL seemed not to have a determining role, an increased HOTAIR expression appeared in recurrent and high-graded tumors associated with poor prognosis. In addition, through genome-wide transcriptome analyses, we observed that HOTAIR-EZH2-complex-regulated genes can efficiently discriminate between non-tumoral, recurrent, and non-recurrent bladder cancer samples. We also observed a significant correlation between EZH2 and HOTAIR expression levels. Using overexpression, knockdown, and pharmacological approaches in bladder cancer cell lines, we also observed that EZH2 regulates HOTAIR expression. Conclusions: Our findings indicate that HOTAIR expression has prognostic value for bladder cancer progression, recurrence, and survival and suggest that HOTAIR plays active roles in modulating the cancer epigenome, becoming an interesting candidate as a target for cancer diagnosis and therapy. The observed HOTAIR regulation by EZH2 and the possibility of modulating EZH2 activity with specific inhibitors open new possible paths to be explored in bladder cancer therapy.
710			_9625 _aInstituto de Investigación imas12
710			_9330 _aServicio de Anatomía Patológica
710			_9303 _aServicio de Oncología Médica
710			_9220 _aServicio de Urología
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599691/ _yAcceso libre
942			_2ddc _cART _n0