000			nab a22 7a 4500
999			_c17082 _d17082
003			PC17082
005			20221122121147.0
008			221122b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_9617 _aFernández Crespo, Jesús _eAlergología
100			_9616 _aRodríguez Rodríguez, Julia _eAlergología
245	0	0	_aImmunoproteomic tools are used to identify masked allergens: Ole e 12, an allergenic isoflavone reductase from olive (Olea europaea) pollen. _h[artículo]
260			_bBiochimica et biophysica acta, _c2015
300			_a1854(12):1871-1880.
500			_aFormato Vancouver: Castro L, Crespo JF, Rodríguez J, Rodríguez R, Villalba M. Immunoproteomic tools are used to identify masked allergens: Ole e 12, an allergenic isoflavone reductase from olive (Olea europaea) pollen. Biochim Biophys Acta. 2015 Dec;1854(12):1871-1880.
501			_aPMID: 26391288
504			_aContiene 50 referencias
520			_aProteins performing important biochemical activities in the olive tree (Olea europaea) pollen have been identified as allergens. One novel 37-kDa protein seems to be associated to the IgE-binding profile of a group of patients suffering allergy to peach and olive pollen. Three previously described olive pollen allergens exhibit very similar molecular mass. Our objective was to identify this allergen by using immunoproteomic approaches. After 2D-electrophoresis and mass spectrometry, peptide sequences from several IgE-binding spots, allowed identifying this new allergen, as well as cloning and DNA sequencing of the corresponding gene. The allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. A prevalence of 33% in the selected population is in contrast to 4%-10% in groups of subjects suffering from pollinosis. Recombinant allergen was produced in Escherichia coli, and deeply characterised. Immunoblotting and ELISA detection as well as inhibition experiments were performed with polyclonal antisera and allergic patients' sera. The recombinant allergen retains the IgE reactivity of its natural counterpart. Close structural and immunological relationships between members of this protein family were supported by their IgG recognition in vegetable species. In summary, Ole e 12 is a minor olive pollen allergen, which gains relevance in patients allergic to peach with olive pollinosis. Proteomic approaches used to analyse this allergen provide useful tools to identify hidden allergens, relevant for several allergic populations and thus complete allergenic panels.
710			_999 _aServicio de Alergología
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc17082.pdf _ySolicitar documento
942			_2ddc _cART _n0