000			nab a22 7a 4500
999			_c17155 _d17155
003			PC17155
005			20230126130320.0
008			230126b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_92429 _aLucía, Alejandro _eInstituto de Investigación i+12
245	0	0	_aMethodological considerations to determine the effect of exercise on brain-derived neurotrophic factor levels. _h[artículo]
260			_bClinical biochemistry, _c2015
300			_a48(3):162-6.
500			_aFormato Vancouver: Pareja Galeano H, Alis R, Sanchís Gomar F, Cabo H, Cortell Ballester J, Gómez Cabrera MC et al. Methodological considerations to determine the effect of exercise on brain-derived neurotrophic factor levels. Clin Biochem. 2015 Feb;48(3):162-6.
501			_aPMID: 25464018
504			_aContiene 44 referencias
520			_aObjectives: Physical exercise up-regulates brain-derived neurotrophic factor (BDNF) in the brain and blood. However, there is yet no consensus about the adequate blood processing conditions to standardize its assessment. We aimed to find a reliable blood sample processing method to determine changes in BDNF due to exercise. Design and methods: Twelve healthy university students performed an incremental cycling test to exhaustion. At baseline, immediately after exercise, and 30 and 60 min of recovery, venous blood was drawn and processed under different conditions, i.e. whole blood, serum coagulated for 10 min and 24 h, total plasma, and platelet-free plasma. BDNF concentration was measured by ELISA. Results: Exercise increased BDNF in whole blood and in serum coagulated for 24 h when corrected by hemoconcentration. We did not find effects of exercise on BDNF in serum coagulated for 10 min or in plasma samples. Plasma shows heterogeneous BDNF values in response to exercise that are not prevented when platelets are eliminated while homogeneous BDNF levels were found in whole blood or serum coagulated for 24 hour samples. Conclusions: In exercise studies, BDNF levels should be adjusted by hemoconcentration. Our data highlight the importance of blood sample selection since the differences between each one affect significantly the BDNF factor changes due to exercise.
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc17155.pdf _ySolicitar documento
942			_2ddc _cART _n0