000			nab a22 7a 4500
999			_c17638 _d17638
003			PC17638
005			20230801125044.0
008			230801b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_91881 _aRey Cerros, Manuel José del _eInstituto de Investigación i+12
100			_92671 _aFaré García, Regina _eInstituto de Investigación i+12
100			_91883 _aUsátegui Corral, Alicia _eInstituto de Investigación i+12
100			_9466 _aGalindo Izquierdo, María _eReumatología
100			_91880 _aCriado Carrasco, Gabriel _eInstituto de Investigación i+12
100			_91010 _aPablos Álvarez, José Luis _eReumatología
245	0	0	_aCD271(+) stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype. _h[artículo]
260			_bArthritis research & therapy, _c2016
300			_a18:66.
500			_aFormato Vancouver: Del Rey MJ, Faré R, Usategui A, Cañete JD, Bravo B, Galindo M et al. CD271(+) stromal cells expand in arthritic synovium and exhibit a proinflammatory phenotype. Arthritis Res Ther. 2016 Mar 15;18:66.
501			_aPMID: 26980374 PMC4791981
504			_aContiene 27 referencias
520			_aBackground: CD271(+) stromal cells (SCs) with multipotent stem cell capacity have been identified in synovial tissues, but their functional significance is unknown. We analyzed the distribution of CD271(+) cells in inflammatory synovial tissues as well as their ex vivo immunomodulatory and inflammatory phenotypes. Methods: CD271 expression was analyzed by immunohistochemistry in synovial tissues and by flow cytometry in primary adherent synovial cell cultures from rheumatoid arthritis (RA), osteoarthritis (OA), and non-inflammatory control tissues. Isolation of CD271(+) synovial SCs was carried out by magnetic cell sorting. Allogeneic T-cell/SC cocultures were performed to analyze the regulatory capacity of these cells on T-cell proliferation and cytokine production. The production of inflammatory mediators was analyzed in cultures of sorted CD271(+)/(-) SCs. The capacity of CD271(+)/(-) SCs to induce inflammatory cell recruitment in vivo was evaluated in subcutaneous implants in immunodeficient mice. Results: CD271(+) SC were detected in non-inflammatory as well as in arthritic synovial tissues with a specific perivascular distribution. CD271(+) SC density was increased in RA and OA compared with normal synovial tissues. T-cell proliferation and cytokine synthesis were similarly modified by CD271(+) and CD271(-) SCs. Sorted CD271(+) SCs from OA synovial tissues released significantly more interleukin (IL)-6, matrix metalloproteinase (MMP)-1, and MMP-3 than CD271(-) SCs. In immunodeficient mice, implants of CD271(+) SCs induced significantly higher myeloid cell infiltration than CD271(-) SCs. Conclusions: Our results demonstrate that CD271(+) perivascular SCs expand in RA and OA synovial tissues. CD271(+) cells showed enhanced proinflammatory properties ex vivo and in vivo, whereas immunoregulatory properties were equivalent in CD271(+) and CD271(-) SC.
710			_9123 _aServicio de Reumatología
710			_9625 _aInstituto de Investigación imas12
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791981/pdf/13075_2016_Article_966.pdf _yAcceso libre
942			_2ddc _cART _n0