000			03036na a2200277 4500
003			H12O
005			20210625062753.0
008			130622s2011 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aGarcía Ruiz, Inmaculada _91460 _eInstituto de Investigación i+12
100			_aGarcía-Consuegra Galiana, Inés _91867 _eInstituto de Investigación i+12
100			_aMuñoz Yagüe, María Teresa _91461 _eAparato Digestivo
100			_9514 _aSolís Herruzo, José Antonio _eAparato Digestivo
245	0	0	_aMelatonin improves mitochondrial respiratory chain activity and liver morphology in ob/ob mice _h[artículo]
260			_bJournal of Pineal Research, _c2011
300			_a51(1):113-123.
500			_aFormato Vancouver: Solís Muñoz P, Solís Herruzo JA, Fernández Moreira D, Gómez Izquierdo E, García Consuegra I, Muñoz Yagüe T, et al. Melatonin improves mitochondrial respiratory chain activity and liver morphology in ob/ob mice. J Pineal Res. 2011;51(1):113-23.
501			_aPMID: 21355880
504			_aContiene 42 referencias
520			_aIn previous studies, we have shown that mitochondrial respiratory chain (MRC) activity is decreased in patients with nonalcoholic steatohepatitis and in ob/ob mice and that peroxynitrite plays a pathogenic role. The present study examined whether melatonin, a peroxynitrite scavenger, prevents: (i) the in vitro effects of peroxynitrite on normal mitochondrial proteins and (ii) the development of nonalcoholic liver disease, MRC dysfunction and proteomic changes found in the mitochondrial complexes from ob/ob mice. We studied MRC activity, assembly of mitochondrial complexes and its subunits in normal mitochondrial proteins exposed to peroxynitrite in the absence and presence of melatonin. The same studies were done in mitochondrial proteins from ob/ob mice untreated and treated with melatonin. Preincubation of mitochondrial proteins from wild-type mice with melatonin prevented 3-tyrosine nitration of these proteins, eliminated the reduction in the MRC activity, the defect in the assembly of mitochondrial complexes and degradation of their subunits induced by peroxynitrite in vitro. Moreover, treatment of ob/ob mice with 10 mg/kg/day melatonin for 12 wk reduced oxidative and nitrosative stress, prevented the loss of MRC activity, protected their complexes and subunits from degradation, and favored assembling of mitochondrial complexes. In addition, this treatment improved fatty liver, decreased hepatic triglyceride concentration and increased apolipoprotein B100 in liver tissue. In conclusion, melatonin prevents the effects of peroxynitrite on mitochondrial proteins in vitro and administration of melatonin to ob/ob mice normalizes liver morphology, mitochondrial dysfunction and assembly of MRC complexes.
710			_9273 _aServicio de Medicina del Aparato Digestivo
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/3/pc342.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c342 _d342