000 | na a22 4500 | ||
---|---|---|---|
999 |
_c4807 _d4807 |
||
003 | PC4807 | ||
005 | 20200129130046.0 | ||
008 | 130622s2013 xxx||||| |||| 00| 0 eng d | ||
040 | _cH12O | ||
041 | _aeng | ||
100 |
_aRuiz Contreras, Jesús _9811 _ePediatría |
||
245 | 0 | 0 |
_aRapid diagnosis of invasive pneumococcal disease in pediatric population. _h[artículo] |
260 |
_bJournal of microbiological methods, _c2013 |
||
300 | _a93(2):116-20. | ||
500 | _aFormato Vancouver: Picazo JJ, Contreras JR, Ríos E, Culebras E, Rodríguez-Avial I, Méndez C et al. Heracles Study Group. Rapid diagnosis of invasive pneumococcal disease in pediatric population. J Microbiol Methods. 2013 May;93(2):116-20. | ||
501 | _aPMID: 23499921 | ||
504 | _aContiene 20 referencias | ||
520 | _aThe aim of this study was to evaluate the Binax NOW immunochromatographic pneumococcal antigen test for the identification of Streptococcus pneumoniae in pleural and cerebrospinal fluids from children with suspected invasive pneumococcal disease. The results were compared with those obtained by PCR. Binax NOW was applied to these samples as recommended by the manufacturer for urine and cerebrospinal samples. Detection of pneumococcal DNA was performed by real-time PCR assay targeting the autolysin gene (lytA). Of the 199 samples analyzed, 131 were positive by both Binax NOW and lytA PCR, and 36 samples were negative by both techniques. Using the real-time PCR as a comparative method to the Binax for the detection of S. pneumoniae, the sensitivity and specificity of Binax NOW was 88% and 72.5%, respectively. Of the 145 positive samples analyzed by Binax NOW, 119 showed intense coloring of the sample line and 26 showed weak intensity. Conventional culture is the most common method in clinical settings, but Binax NOW is an easier and faster test for identifying S. pneumoniae in pleural and cerebrospinal fluids from children with suspected invasive pneumococcal disease. | ||
710 |
_9446 _aServicio de Pediatría-Neonatología |
||
856 |
_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/4/pc4807.pdf _ySolicitar documento |
||
942 |
_n0 _2ddc _cART |