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| 003 | H12O | ||
| 005 | 20210625062754.0 | ||
| 008 | 130622s2011 xxx||||| |||| 00| 0 eng d | ||
| 040 | _cH12O | ||
| 041 | _aeng | ||
| 100 |
_aAgulló Ortuño, María Teresa _91791 _eOncología Médica |
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| 245 | 0 | 0 |
_aProtein phosphorylation analysis in archival clinical cancer samples by shotgun and targeted proteomics approaches _h[artículo] |
| 260 |
_bMolecular Biosystems, _c2011 |
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| 300 | _a7(8):2368-2374. | ||
| 500 | _aFormato Vancouver: Gámez Pozo A, Sánchez Navarro I, Calvo E, Díaz E, Miguel Martín M, López R, et al. Protein phosphorylation analysis in archival clinical cancer samples by shotgun and targeted proteomics approaches. Mol Biosyst. 2011;7(8):2368-74. | ||
| 501 | _aPMID: 21617801 | ||
| 504 | _aContiene 35 referencias | ||
| 520 | _aProtein phosphorylation affects most eukaryotic cellular processes and its deregulation is considered a hallmark of cancer and other diseases. Phosphoproteomics may enable monitoring of altered signaling pathways as a means of stratifying tumors and facilitating the discovery of new drugs. Unfortunately, the development of molecular tests for clinical use is constrained by the limited availability of fresh frozen, clinically annotated samples. Here we report phosphopeptide analysis in human archival formalin-fixed, paraffin-embedded (FFPE) cancer samples based on immobilized metal affinity chromatography followed by liquid chromatography coupled with tandem mass spectrometry and selected reaction monitoring techniques. Our results indicate the equivalence of detectable phosphorylation rates in archival FFPE and fresh frozen tissues. Moreover, we demonstrate the applicability of targeted assays for phosphopeptide analysis in clinical archival FFPE samples, using an experimental workflow suitable for processing and analyzing large sample series. This work paves the way for the application of shotgun and targeted phosphoproteomics approaches in clinically relevant studies using archival clinical samples. | ||
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_9625 _aInstituto de Investigación imas12 |
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_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/5/pc592.pdf _ySolicitar documento |
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_n0 _2ddc _cART |
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_c592 _d592 |
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