| 000 | 02041nab a2200277 4500 | ||
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| 999 |
_c6409 _d6409 |
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| 003 | PC6409 | ||
| 005 | 20210625062803.0 | ||
| 008 | 130622s2014 xxx||||| |||| 00| 0 eng d | ||
| 040 | _cH12O | ||
| 041 | _aeng | ||
| 100 |
_aAntequera, Desiree _92134 _eInstituto de Investigación i+12 |
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| 100 |
_aCarro Díaz, Eva _91777 _eInstituto de Investigación i+12 |
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| 100 |
_aPérez González, Rocío _91931 _eInstituto de Investigación i+12 |
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| 245 | 0 | 0 |
_aLeptin gene therapy attenuates neuronal damages evoked by amyloid-beta and rescues memory deficits in APP/PS1 mice. _h[artículo] |
| 260 |
_bGene therapy, _c2014 |
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| 300 | _a21(3):298-308. | ||
| 500 | _aFormato Vancouver: Pérez-González R, Alvira-Botero MX, Robayo O, Antequera D, Garzón M, Martín-Moreno AM et al. Leptin gene therapy attenuates neuronal damages evoked by amyloid-β and rescues memory deficits in APP/PS1 mice. Gene Ther. 2014 Mar;21(3):298-308. | ||
| 501 | _aPMID: 24430238 | ||
| 504 | _aContiene 61 referencias | ||
| 520 | _aThere is growing evidence that leptin is able to ameliorate Alzheimer's disease (AD)-like pathologies, including brain amyloid-beta (Abeta) burden. In order to improve the therapeutic potential for AD, we generated a lentivirus vector expressing leptin protein in a self-inactivating HIV-1 vector (HIV-leptin), and delivered this by intra-cerebroventricular administration to APP/PS1 transgenic model of AD. Three months after intra-cerebroventricular administration of HIV-leptin, brain Abeta accumulation was reduced. By electron microscopy, we found that APP/PS1 mice exhibited deficits in synaptic density, which were partially rescued by HIV-leptin treatment. Synaptic deficits in APP/PS1 mice correlated with an enhancement of caspase-3 expression, and a reduction in synaptophysin levels in synaptosome preparations. Notably, HIV-leptin therapy reverted these dysfunctions. Moreover, leptin modulated neurite outgrowth in primary neuronal cultures, and rescued them from Abeta42-induced toxicity. All the above changes suggest that leptin may affect multiple aspects of the synaptic status, and correlate with behavioral improvements. Our data suggest that leptin gene delivery has a therapeutic potential for Abeta-targeted treatment of mouse model of AD. | ||
| 710 |
_9625 _aInstituto de Investigación imas12 |
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| 856 |
_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/6/pc6409.pdf _ySolicitar documento, |
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| 942 |
_n0 _2ddc _cART |
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